KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD45-Brilliant Violet 605 (30-F11) | BioLegend | Cat# 103140, RRID: AB_2562342 |
| CD45-Brilliant Violet 785 (30-F11) | BioLegend | Cat# 103149, RRID: AB_2564590 |
| CD3ε-PerCP-Cy5.5 (145-2C11) | BioLegend | Cat# 100328, RRID: AB_893318 |
| CD3ε-Brilliant Violet 421 (145-2C11) | BioLegend | Cat# 100336, RRID: AB_11203705 |
| CD5-PerCP-Cy5.5 (53-7.3) | BioLegend | Cat# 100624, RRID: AB_2563433 |
| CD8α-PerCP-Cy5.5 (53-6.7) | BioLegend | Cat# 100734, RRID: AB_2075238 |
| NK1.1-PerCP-Cy5.5 (PK136) | BioLegend | Cat# 108728, RRID: AB_2132705 |
| CD11c-APC-Fire750 (N418) | BioLegend | Cat# 117352, RRID: AB_2572124 |
| CD11c-PE-Cy7 (N418) | BioLegend | Cat# 117318, RRID: AB_493568 |
| CD11b-APC-Fire750 (M1/70) | BioLegend | Cat# 101262, RRID: AB_2572122 |
| CD11b-PE-Dazzle 594 (M1/70) | BioLegend | Cat# 101256, RRID: AB_2563648 |
| B220-APC-Fire750 (RA3-6B2) | BioLegend | Cat# 103260, RRID: AB_2572109 |
| CD19-PE-Cy7 (eBio1D3) | ThermoFisher | Cat# 25-0193-82, RRID: AB_657663 |
| CD19-Brilliant Violet 605 (6D5) | BioLegend | Cat# 115540, RRID: AB_2563067 |
| TCRβ-APC-Fire750 (H57-597) | BioLegend | Cat# 109246, RRID: AB_2629697 |
| CD127-PE-Cy5 (A7R34) | BioLegend | Cat# 135016, RRID: AB_1937261 |
| CD90.2-Alexa Fluor 700 (30-H12) | BioLegend | Cat# 105320, RRID: AB_493725 |
| F4/80-APC (BM8) | BioLegend | Cat# 123116, RRID: AB_893481 |
| Ly6G-Alexa Fluor 700 (RB6-8C5) | BioLegend | Cat# 108422, RRID: AB_2137487 |
| Siglec-F-PE (E50-2440) | BD Biosciences | Cat# 552126, RRID: AB_394341 |
| SIRPα-Alexa Fluor 647 (P84) | BioLegend | Cat# 144028, RRID: AB_2721301 |
| XCR1-FITC (ZET) | BioLegend | Cat# 148210, RRID: AB_2564366 |
| CD4-BUV395 (GK1.5) | BD Biosciences | Cat# 563790, RRID: AB_2738426 |
| CD4-Brilliant Violet 650 (RM4-5) | BioLegend | Cat# 100546, RRID: AB_2562098 |
| MHCII-Brilliant Violet 650 (M5/114.15.2) | BioLegend | Cat# 107641, RRID: AB_2565975 |
| CD45.1-Brilliant Violet 421 (A2) | BioLegend | Cat# 110732, RRID: AB_2562563 |
| CD44-APC (IM7) | BioLegend | Cat# 103012, RRID: AB_312963 |
| NKp46-PE (29A1.4) | BioLegend | Cat# 137604 RRID: AB_2235755 |
| CD25-PE-Cy7 (PC61.5) | BioLegend | Cat# 102016, RRID: AB_312865 |
| CCR6-Brilliant Violet 421 (29-2L17) | BioLegend | Cat# 129818, RRID: AB_11219003 |
| KLRG1-APC (2F1) | BioLegend | Cat# 138412 RRID: AB_10641560 |
| CD80-PE-eFluor 610 (16-10A1) | BioLegend | Cat# 104738, RRID: AB_2564175 |
| CD86-BUV395 (GL1) | BD Biosciences | Cat# 564199, RRID: AB_2738664 |
| CCR7-PE (4B12) | BioLegend | Cat# 120106, RRID: AB_389358 |
| CD69-PerCP-Cy5.5 (H1.2F3) | ThermoFisher | Cat# 45-0691-82, RRID: AB_1210703 |
| CCR4-PE (2G12) | BioLegend | Cat# 131204, RRID: AB_1236367 |
| CCR8-FITC (SA214G2) | BioLegend | Cat# 150314, RRID: AB_2629604 |
| CXCR3-Brilliant Violet 605 (CXCR3-173) | BioLegend | Cat # 126523, RRID: AB_2561353 |
| Foxp3-Alexa Fluor 700 (FJK-16 s) | ThermoFisher | Cat# 56-5773-82, RRID: AB_1210557 |
| RORγt-PE (B2D) | ThermoFisher | Cat# 12-6981-82, RRID: AB_10807092 |
| RORγt-PE-eFluor610 (B2D) | ThermoFisher | Cat# 61-6981-82, RRID: AB_2574650 |
| RORγt-Brilliant Violet 650 (Q31-378) | BD Biosciences | Cat# 564722, RRID: AB_2738915 |
| GATA-3-BUV395 (L50-823) | BD Biosciences | Cat# 565448, RRID: AB_2739241 |
| T-bet-Brilliant Violet 421 (4B10) | BD Biosciences | Cat# 644816, RRID: AB_10959653 |
| Aire-eFluor 660 (5H12) | ThermoFisher | Cat# 50-5934-80, RRID: AB_2574256 |
| IL-13-Alexa Fluor 488 (eBio13A) | ThermoFisher | Cat# 53-7133-82, RRID: AB_2016708 |
| IL-2-PE (JES6-5H4) | BioLegend | Cat# 503808, RRID: AB_315302 |
| IL-22-Alexa Fluor 647 (IL22JOP) | ThermoFisher | Cat# 17-7222-82, RRID: AB_10597583 |
| GM-CSF-FITC (MP1-22E9) | BioLegend | Cat# 505404, RRID: AB_315380 |
| IL-17A-BUV395 (TC11-18H10) | BD Biosciences | Cat# 565246, RRID: AB_2722575 |
| CD3-PE-Cy7 (UCHT1) | BioLegend | Cat# 300420, RRID: AB_439781 |
| CD4-PE-Dazzle 594 (OKT4) | BioLegend | Cat# 317448, RRID: AB_2565847 |
| CD5-PE-Cy7 (UCHT2) | BioLegend | Cat# 300622, RRID: AB_2275812 |
| CD11b-Alexa Fluor 700 (M1/70) | BioLegend | Cat# 101222, RRID: AB_493705 |
| CD11c-Alexa Fluor 700 (3.9) | BioLegend | Cat# 301648, RRID: AB_2819923 |
| CD14-Alexa Fluor 700 (HCD14) | BioLegend | Cat# 325614, RRID: AB_830687 |
| CD19-Alexa Fluor 700 (HIB19) | BioLegend | Cat# 302226, RRID: AB_493751 |
| HLA-DR-BUV395 (L203) | BD Biosciences | Cat# 752492, RRID: N/A |
| CRTH2-APC (BM16) | BioLegend | Cat# 350110, RRID: AB_11203707 |
| CD127-APC-eFluor 780 (eBioRDR5) | ThermoFisher | Cat# 47-1278-42, RRID: AB_1548674 |
| FcεRI-PE-Cy7 (AER-37) | BioLegend | Cat# 334620, RRID: AB_10575314 |
| c-kit-PerCP-Cy5.5 (104D2) | BioLegend | Cat# 313214, RRID: AB_2131443 |
| CD45-Brilliant Violet 605 (HI30) | BioLegend | Cat# 304042, RRID: AB_2562106 |
| NKp44-PE (P44-8) | BioLegend | Cat# 325108, RRID: AB_756100 |
| secondary antibodies against murine IgG1-HRP | BD Biosciences | Cat# 559626 |
| secondary antibodies against murine IgE | ThermoFisher | Cat#MA5-16781 |
| Anti-mouse IL-17A, purified | ThermoFisher | Cat# 14-7175-81 |
| Anti-mouse IL-17A, biotin | ThermoFisher | Cat# 13-717781 |
| Anti-mouse IL-13, purified | ThermoFisher | Cat# 14-7133-81 |
| Anti-mouse IL-13, biotin | ThermoFisher | Cat# 13-7135-81 |
| purified anti-mouse RORγt (AFKJS-9) | ThermoFisher | Cat# 14-6988-82, RRID: AB_1834475 |
| biotinylated anti-mouse CD3ε (eBio500A2) | ThermoFisher | Cat# 13-0033-82, RRID: AB_842774 |
| donkey anti-rat-IgG-FITC | Jackson ImmunoResearch | Cat# 712-097-003, RRID: AB_2340655 |
| CD127-eFluor 660 (A7R34) | ThermoFisher | Cat# 50-1271-82, RRID: AB_11219081 |
| donkey anti-rabbit-IgG-Alexa Fluor 488 | ThermoFisher | Cat# A-21206, RRID: AB_2535792 |
| Biological samples | ||
| Human lung and lung mediastinal lymph node tissue samples | LiveOnNY | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Papain | Worthington | Cat#LS003126 |
| 2W1S peptide | GenScript | Peptide sequence: EAWGALANWAVDSA |
| I-A(b) mouse 2W1S tetramer, PE conjugated | NIH Tetramer Core Facility | N/A |
| house dust mite protein extract | Greer Laboratories | Cat# XPB82D3A2.5 |
| Naive CD4+ T Cell Isolation Kit, mouse | Miltenyi Biotec | Cat# 130-104-453 |
| Pentobarbital | American Pharmaceutical Partners | N/A |
| Liberase TL | Sigma | Cat# 5401020001 |
| Collagenase D | Sigma | Cat# 11088882001 |
| ACK lysis buffer | VWR | Cat# 12002-070 |
| RPMI 1640 with L-glutamine | Corning | Cat#10-040-CV |
| trypsin inhibitor | Life Technologies | Cat# 17075-029 |
| DNase I | Sigma | Cat# D5025 |
| Collagenase II | ThermoFisher | Cat#17101015 |
| TMB peroxidase substrate | ThermoFisher | Cat# 00-4201-56 |
| House dust mite milled raw materials | Greer Laboratories | N/A |
| House dust mite milled raw materials | AirMid Healthgroup, | N/A |
| House dust mite milled raw materials | HollisterStier | N/A |
| House dust mite milled raw materials | CiTeq Biologics | N/A |
| Paraformaldehyde (4% in PBS) | bioWORLD | Cat# 30450002-1 |
| Tissue-Tek OCT compound | Bayer Healthcare | N/A |
| Streptavidin-Alexa Fluor 555 | ThermoFisher | Cat#S21381 |
| DAPI | ThermoFisher | Cat#D1306 |
| ProLong Gold Antifade Mountant | ThermoFisher | Cat# P36934 |
| Critical commercial assays | ||
| Live/Dead Fixable Aqua Dead Cell Stain Kit | Thermo Fisher Scientific | Cat# L34957 |
| Foxp3 transcription factor staining buffer set | Thermo Fisher Scientific | Cat# 00-5523-00 |
| IL-2 fluorokine kit | R&D Systems | Cat# BT202-025 |
| Deposited data | ||
| 16S sequencing of HDM from various vendors | This paper | BioProject ID PRJNA762346 |
| Metagenomic sequencing of HDM from various vendors | This paper | BioProject ID PRJNA762364 |
| Experimental models: Organisms/strains | ||
| C57BL/6J mice | Jackson Laboratory | RRID:IMSR_JAX:000664 |
| Rorccre mice | Dr. Gerard Eberl | N/A |
| H2-Ab1fl/fl mice | Jackson Laboratory | RRID:IMSR_JAX:013181 |
| 1-DEPβ mice | Dr. Bart N. Lambrecht | N/A |
| Ffar2−/− mice | Dr. M van den Brink | N/A |
| Software and algorithms | ||
| Flexivent | Scireq | https://www.scireq.com/flexivent |
| FlowJo V1 0.8.0 | TreeStar | https://www.flowjo.com/solutions/flowjo/downloads |
| FACS Diva | BD Biosciences | https://www.bdbiosciences.com/en-us/products/software/instrument-software/bd-facsdivasoftware#Overview |
| Cutadapt | Caporaso et al., 2011 | https://github.com/marcelm/cutadapt |
| Qiime2 dada2 plugin V 1.10.0 | Caporaso et al., 2011 | https://github.com/qiime2/q2-dada2 |
| GraphPad Prism V 9.2.0 | GraphPad | https://www.graphpad.com |
| ImageJ | NIH | https://imagej.nih.gov/ij/download.html |