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. 2021 Nov 17;17(11):e1009921. doi: 10.1371/journal.pgen.1009921

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© 2021 Girard et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) LD staining of whole-mount retina from flies with photoreceptor neuron-specific (Rh1-GAL4) expression of dPlin1::GFP and LacZ-RNAi (control), dFatp-RNAi, or mdy-RNAi. LDs are in green (BODIPY) and photoreceptor rhabdomeres are in magenta (phalloidin-rhodamine). Scale bar, 10 μm. (B) Quantification of LD area from the images shown in (A). Mean ± SD. n.s., not significant by ANOVA. (C) LD staining of whole-mount retinas from flipase mediated FRT dFatp[k10307] mutant clone in conjunction with expression of dPlin1::GFP in photoreceptor (Rh1-GAL4). LDs are in green (BODIPY), wild type photoreceptors are in magenta (FRT40A-Tomato[ninaC]) and rhabdomeres are in grey (phalloidin-rhodamine). LDs are visible in photoreceptors lacking dFatp (mutant clone surrounded by dashed line). Scale bar, 10 μm. (D, E) whole-mount retinas from mdy^[QX25] heterozygous and homozygous flies expressing dPlin1::GFP in photoreceptor (Rh1-GAL4). (D) LDs are in green (BODIPY), and rhabdomeres are in magenta (phalloidin-rhodamine). (E) Photoreceptor plasma membranes are in cyan (anti-Na⁺/K⁺ ATPase) and rhabdomeres are in magenta (phalloidin-rhodamine). (F) LD staining of whole-mount retinas from flies expressing bmm-RNAi in photoreceptor (Rh1-GAL4) or retina glia (54C-GAL4). LDs are in green (BODIPY), and rhabdomeres are in magenta (phalloidin-rhodamine). Scale bar, 10 μm. (G) LD staining of whole-mount retinas from flies expressing dPlin2::GFP in conjunction with RNAi targeting LacZ or bmm in photoreceptor (Rh1-GAL4). LDs are in green (BODIPY), and rhabdomeres are in magenta (phalloidin-rhodamine). Scale bar, 10 μm. (H) Quantification of LD area from the images shown in (G). Mean ± SD. n.s., not significant by t-test. (I) Immunostaining of whole-mount retinas from homozygous bmm mutant flies. Photoreceptor plasma membranes are in cyan (anti-Na⁺/K⁺ ATPase) and rhabdomeres are in magenta (phalloidin-rhodamine). Scale bar, 10 μm.