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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Dev Cell. 2019 Nov 14;51(5):617–631.e3. doi: 10.1016/j.devcel.2019.10.008

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and Virus Strains
E. coli OP50 Caenorhabditis Genetics Center https://cgc.umn.edu/strain/OP50
Chemicals, Peptides, and Recombinant Proteins
Histamine dihydrochloride Sigma-Aldrich Cat# H7250-5G
levamisole Sigma-Aldrich Cat#1359302
all trans-Retinal Sigma-Aldrich Cat# R2500
Alt-R® S.p.Cas9 Nuclease V3 IDT Cat#1081059
Histoacryl® Blue B∣Braun Cat# TS1050044FP-SD
Critical Commercial Assays
Phusion High-Fidelity DNA polymerase New England Cat # M0530S
Gibson Assembly® Master Mix New England Cat# E2611S
Q5® Site-Directed Mutagenesis Kit New England Cat# E0554S
Experimental Models: Organisms/Strains
C. elegans strains This paper see Table S1
Oligonucleotides
SgRNA sequences and CRISPR repair templates This paper see Table S3
Alt-R® CRISPR-Cas9 tracrRNA IDT Cat# 1072532
Recombinant DNA
Punc-17::UNC-8 cDNA This paper N/A
Pser2prom3::UNC-8 cDNA This paper N/A
Punc-17::DEL-1 cDNA This paper N/A
Pser2prom3::DEL-1 cDNA This paper N/A
Pser2prom3::MEC-10 cDNA This paper N/A
Pser2prom3::NLP-12 cDNA::SL2::mcherry This paper N/A
Ptwk-16::NLP-12 cDNA::sl2::mcherry This paper N/A
Pser2prom3::UNC-8 cDNA::mcherry This paper N/A
Pser2prom3::DEL-1 cDNA::GFP This paper N/A
Pser2prom3::DEGT-1::cDNA::mcherry This paper N/A
pBHC1084 N/A N/A
pJW1259 Ward, 2015 Cat# 61251
Software and Algorithms
ImageJ NIH https://imagej.nih.gov/ij/
Prism 6 GraphPad https://www.graphpad.com/scientific-software/prism/