Bacterial and Virus Strains |
E. coli OP50 |
Caenorhabditis Genetics Center |
https://cgc.umn.edu/strain/OP50
|
Chemicals, Peptides, and Recombinant Proteins |
Histamine dihydrochloride |
Sigma-Aldrich |
Cat# H7250-5G |
levamisole |
Sigma-Aldrich |
Cat#1359302 |
all trans-Retinal |
Sigma-Aldrich |
Cat# R2500 |
Alt-R® S.p.Cas9 Nuclease V3 |
IDT |
Cat#1081059 |
Histoacryl® Blue |
B∣Braun |
Cat# TS1050044FP-SD |
Critical Commercial Assays |
Phusion High-Fidelity DNA polymerase |
New England |
Cat # M0530S |
Gibson Assembly® Master Mix |
New England |
Cat# E2611S |
Q5® Site-Directed Mutagenesis Kit |
New England |
Cat# E0554S |
Experimental Models: Organisms/Strains |
C. elegans strains |
This paper |
see Table S1
|
Oligonucleotides |
SgRNA sequences and CRISPR repair templates |
This paper |
see Table S3
|
Alt-R® CRISPR-Cas9 tracrRNA |
IDT |
Cat# 1072532 |
Recombinant DNA |
Punc-17::UNC-8 cDNA |
This paper |
N/A |
Pser2prom3::UNC-8 cDNA |
This paper |
N/A |
Punc-17::DEL-1 cDNA |
This paper |
N/A |
Pser2prom3::DEL-1 cDNA |
This paper |
N/A |
Pser2prom3::MEC-10 cDNA |
This paper |
N/A |
Pser2prom3::NLP-12 cDNA::SL2::mcherry |
This paper |
N/A |
Ptwk-16::NLP-12 cDNA::sl2::mcherry |
This paper |
N/A |
Pser2prom3::UNC-8 cDNA::mcherry |
This paper |
N/A |
Pser2prom3::DEL-1 cDNA::GFP |
This paper |
N/A |
Pser2prom3::DEGT-1::cDNA::mcherry |
This paper |
N/A |
pBHC1084 |
N/A |
N/A |
pJW1259 |
Ward, 2015
|
Cat# 61251 |
Software and Algorithms |
ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
Prism 6 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|