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. 2021 Nov 17;8:742877. doi: 10.3389/fvets.2021.742877

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Copyright © 2021 van Dixhoorn, te Beest, Bolhuis, Parmentier, Kemp, van Mourik, Stockhofe-Zurwieden, van Reenen and Rebel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental process and timeline of the measurements: piglets were observed from birth until 55 days of age. Enriched housed pigs experienced social enrichment from day 13 until weaning; coping strategy was assessed at day 17. Piglets were weaned at day 31 and infected with porcine reproductive and respiratory syndrome virus (PRRSV) at day 44 and with Actinobacillus pleuropneumoniae at day 52 and euthanized at day 55. Body weight was measured weekly; rectal temperature was measured at days 40 and 44; behavior and skin lesions were assessed at days 30, 32, 38, and 40. Natural antibodies (NAbs) were measured at days 38 and 44; white blood cell (WBC) at day 44 and histological lung lesion scores were assessed after day 55.