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. 2021 Nov 17;8:760487. doi: 10.3389/fvets.2021.760487

Figure 1.

Figure 1

Pairwise Variance (V-score) (A,B) and Average expression stability M (C) of miRNAs included in geNorm analyses to determine the optimal number of miRNAs for data normalization. Results when including 5 (A; miR-23a-3p, miR-191-5p, miR-222-3p, miR-423-5p and miR-425-5p) and when including 16 (B,C; miR-7-5p, miR-16-5p, miR-18a-5p, miR-21-5p, miR-23a-3p, miR-122-5p, miR-126-5p, miR-191-5p, miR-194-5p, miR-222-3p, miR-223-3p, miR-320a-3p, miR-375-3p, miR-423-5p, miR-425-5p and miR-483-3p) miRNAs. Left panels (EDTA plasma) include samples from healthy dogs (n = 6) and from dogs with pituitary-dependent hypercortisolism (n = 19, pre-op and post-op samples); middle panels (serum) include samples from healthy dogs (n = 6) and from dogs with cortisol-secreting adrenocortical tumors (n = 26); right panels (all) include both EDTA plasma and serum samples (n = 76).