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. 2021 Nov 17;9:774486. doi: 10.3389/fchem.2021.774486

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Copyright © 2021 Wan, Zhao, Deng, Chen, Xi and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A,B) Steady-state kinetic assay of NGQDs, in which the reaction velocity is determined through oxidation of TMB based on absorption at 652 nm with varying concentrations of (A) TMB or (B) H₂O₂. (C,D) Double-reciprocal plots of NGQD activity obtained using Michaelis–Menten model at a fixed concentration of H₂O₂ (c, 6.6 mM) or TMB (d, 0.5 mM) versus various concentrations of TMB (C) or H₂O₂ (D).