. 2021 Nov 18;10(1):2128–2140. doi: 10.1080/22221751.2021.1999177

Table 3.

Genotypic and phenotypic characterization of selected strains from previously described lineages and/or clades possessing a pFv-related plasmid.

Lineage/clade^a	Selected strain	Isolation data^b		API20E profile^c	PCR for^d:					Serology^e
Lineage/clade^a	Selected strain	Source	Year	API20E profile^c	species	pv. piscis	clade E	ftbp	PHH	SerE	SerA	SerI	SerO	SerT
L1-clade A	yb158	Healthy tilapia	2005	5146105	+	+	-	+	+	-	-	-	-	+
	V246	Human blood	2005	5146105	+	+	-	+	+	-	-	-	-	+
L3	12	Human blood	1996	4146005	+	+	-	+	+	-	-	-	+	-
L5	V252	Human blood	2004	5346005	+	+	-	+	-	-	-	-	-	-

^{^a}

Phylogenetic lineage determined by Roig et al [11]. L3 and L5 are clonal complexes.

^{^b}

Data of isolation for the selected strain

^{^c}

Probability of identification of V. vulnificus: 4146005, 75.5%; 5146105, 99.3%.

^{^d}

The target genes for PCR were: vvhA, species; fpcrp, pv. piscis (PCR designed in this work); seq61, zoonotic clade E [21]; ftbp, fish transferrin binding protein [12]; a pilF polymorphism, public health hazard (PHH) [22]. Discrimination is based on the amplification of a variable region located within the gene pilF resulting in a 338 bp fragment.

^{^e}

+, AgO agglutination in less than 1 min and ELISA titter (the reciprocal of the highest dilution of the antiserum giving OD two times higher than that of the negative control) higher than 60,000. -, no agglutination and ELISA titer lower than 15,000.