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Sample flow and experimental approach used in this study. The chart illustrates the methods for screening non-structural protein 1 (NS1) and anti-DENV immunoglobulin G (IgG) and IgM using a commercial OnSite Duo Dengue Ag-IgG/IgM rapid test (CTK BIOTECH Inc, CA, USA), detection of DENV RNA by conventional reverse transcription polymerase chain reaction (RT-PCR) and genetic characterization after sequencing and phylogenetic analysis
