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. 2021 Dec 2;198:113857. doi: 10.1016/j.bios.2021.113857

Fig. 1.

Fig. 1

Specific identification of SARS-CoV-2 mutations using CRISPR/Cas12a cis-cleavage and trans-cleavage by MT-specific crRNA-M and WT-specific crRNA-W. (A) Schematic representation of Cas12a/crRNA-W and Cas12a/crRNA-M to differentiate MT-DNA from WT-DNA. (B, C, D) in vitro cleavage assay of N501Y, D614G and 69/70 deletion targets using Cas12a/crRNA-W and Cas12a-crRNA-M. WT: wild-type DNA; MT: mutant DNA; NC: negative control. (E, F, G) Identification of N501Y, D614G and 69/70 deletion targets through CRISPR/Cas12a trans-cleavage of the fluorescence reporter.