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. 2021 Dec 2;198:113857. doi: 10.1016/j.bios.2021.113857

Fig. 3.

Fig. 3

Sensitivity of SARS-CoV-2 mutant RNA identification using the RT-CORDS fluorescence reporting system. (A, B) Sensitivity of synthetic Y501-MT RNA detection using the RT-CORDS fluorescence assay. (A) Fluorescence kinetics of FQ-ssDNA reporter transcleaved by Cas12a using various Y501-MT RNA concentrations. (B) Background-corrected RFU of Y501-MT detection using various Y501-MT RNA concentrations at 10 min. (C, D) Sensitivity of synthetic 69/70-MT RNA detection using the RT-CORDS fluorescence assay. (E, F) Sensitivity of synthetic G614-MT RNA detection using the RT-CORDS fluorescence assay. In all figures error bars represent the means ± standard deviation (SD) from replicates (n = 3).