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. 2021 Nov 17;9:710382. doi: 10.3389/fped.2021.710382

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Copyright © 2021 Yu, Lv, Gao, Shi, Sheng, Zheng, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of macrophages on stimulations of macrophages (M1, 20 ng/ml LPS and 50 ng/ml IFN-γ; M2, 20 ng/ml IL-4). (A) M1 macrophages (scale bars = 50 μm); (B) M2 macrophages (scale bar = 50 μm). The changes of inflammation factors in RAW264.7 and BMDM in different mediums and different times, and mRNA levels of iNOS (C), TNF-α (D), IL-1β (E), IL-10 (F), Arg-1 (H), and protein YM-1 (G). The mRNA levels of in RAW264.7 macrophages with and without hydrogen, iNOS (I), TNF-α (J), IL-1β (K), IL-10 (L), YM-1 (M), Arg-1 (N), ^* M1 or M2 vs. M0, ^# M1+H₂ or M2+H₂ vs. M0 (^*,^# p < 0.05, ^** p < 0.01, ^*** p < 0.001).