Figure 1.

Irf8^R294C mutation leads to alteration in pDC transcriptome. (A) Plot showing principal component analysis of six samples; distance between the samples depicts the difference in gene expression among the two sets (IRF8^R294C samples 1 and 3 are overlapping, hence appearing as a single dot). (B) MA plot presenting differential gene expression, indicating upregulated (in red) and downregulated (in green) genes in IRF8^WT. (C) Volcano plot displaying the differential gene expression between the two groups of samples with upregulated and downregulated genes in IRF8^WT illustrated in red and green, respectively. (D) KEGG pathway analysis of upregulated genes in IRF8^WT; the outer circle represents top upregulated pathways, and the inner circle indicates corresponding –log₁₀FDR value of respective KEGG pathway. (E) Heatmap depicting relative expression of top 50 genes across six samples with red and green color denoting upregulated and downregulated genes in IRF8^WT, respectively.