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Impaired type I IFN production in IRF8R294C pDCs leads to impairment in induction of ISGs. Transcript analysis of Irf7, Oas3, Oasl2, Oas1g, Oas1a, Ifit3, and Oas2 by qRT-PCR in FLDC cultures from IRF8WT and IRF8R294C mice stimulated with CpG (A) or infected with NDV (B) at indicated time points. Data (A, B) are representative of three independent experiments with error bar representing ± SEM.
