Figure 5.

IRF8^R294C pDCs are defective in activation upon immune challenges. FLDCs from IRF8^WT and IRF8^R294C mice were kept unstimulated or stimulated with CpG or infected with NDV, and CD11c⁺SiglecH⁺ pDCs were analyzed for expression of costimulatory molecules. Flow cytometric analysis of expression of (A) CD40, (C) CD86, (E) CD80 markers, and quantitation of expression of the respective markers (B, D, F) are shown. Data (A, C, E) are representative of three independent experiments. Data (B, D, F) are mean of three independent experiments with error bar representing + SEM and *p < 0.05, **p < 0.01, ***p < 0.001. p value obtained from Student’s t test.