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. 2021 Nov 20;11(22):e4221. doi: 10.21769/BioProtoc.4221

Figure 1. Preparation for MRI.

Figure 1.

A. Sample preparation: Remove the tissues outside of the skull carefully without damaging the eyeballs (top panel). Place the brain in a 5ml syringe with small pieces of zip-ties to fix its position (bottom panel). B-C. Remove air from the syringe: Connect the syringe to a loosely tied vacutainer filled with Fomblin® (shown in C) and place in the vacuum chamber (shown in B). Remove the vacutainer and turn on the vacuum for 30 min to remove air bubbles. Push out the remaining air after vacuum and seal the top by tightening the vacutainer. D. Place the sample in a manufacturer-made sample holder designed for the cryogenic probe. D’. A zoom-in view of the sample. E. Insert the sample holder into the magnet bore of the magnet. E’. A zoom-in view of the holder (indicated by the white arrow). F. Three orthogonal plane images acquired using the Localizer protocol on a 7 Tesla Bruker preclinical MRI system.