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. 2021 Nov 24;2021:4468625. doi: 10.1155/2021/4468625

Figure 4.

Figure 4

Chemerin activated CMKLR1 and aggravated high glucose-induced cell injury in ARPE-19 cells. (a) ARPE-19 cells were exposed to different concentrations of D-glucose (0–100 mM) treatment for 24 h, and then cell viability was evaluated by MTT. 30 mM was taken as high glucose (HG) concentration to stimulate ARPE-19 cells for further studies. (b) ARPE-19 cells were exposed to different concentrations of recombinant human chemerin and 30 mM HG for 24 h, and then cell viability was evaluated by MTT. 10 nM recombinant human chemerin was chosen for further experiments. N = 6 for each group. After 24 exposure, RT-qPCR was used to measure the mRNA levels of CMKLR1, and western blot was used to measure the protein levels of CMKLR1 (c–e). N = 3 for each group. #p < 0.05, ##p < 0.01, and ###p < 0.001 compared to control, @p < 0.05 and @@@p < 0.001 compared to HG group. One-way ANOVA followed by Dunnett's T3 multiple comparisons test.