Table 1.
List of divergent primers designed for RT-PCR validation of circRNAs.
| circRNAs | Primer sequence (5′-3′) | Annealing temperature (°C) | Size (bp) |
|---|---|---|---|
| circ-AABR | Forward: TGGGAAGAAAGGGGTGAC | 60 | 265 |
| Reverse: GTAAAGGGTACTAGAGCC | |||
| circ-Nf1x | Forward: TGTGCGTCCAGCCACATC | 60 | 310 |
| Reverse: AACTCGGGCCGGATGTCT | |||
| circ-Homer1 | Forward: GCAAAGGAGAAGTCGCAG | 65 | 350 |
| Reverse: TGCCTTTGAGCCGTCTAG | |||
| circ-Chsy1 | Forward: TGTACACCACCCACGAGGAT | 58 | 300 |
| Reverse: CGATCTCCCTTGATGTAGAC | |||
| circ-Snap | Forward: ATGTGCATCCAAATGAGCTA | 60 | 178 |
| Reverse: TCAAAGAGGGTATCCGCCAT | |||
| circ-Chd | Forward: AAATCGGAGCAGACAAGAACC | 55 | 233 |
| Reverse: GGCTCTCAGAAGATTCCGAAC | |||
| circ-Wdr-F | Forward: CAGTTTCTCACCCACGGAT | 65 | 198 |
| Reverse: GCCGCTTATAGAACAACTGTCG | |||
| circ-int3193 | Forward: CCCTACAAGAAGCACCATCGT | 58 | 360 |
| Reverse: AGGCAGTGGGTGTTGTGACT | |||
| circ-Adam | Forward: GAGTACGTGTGGATCACAGG | 60 | 302 |
| Reverse: GGAGGTTTGTCGAGGACTTC |