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. 2021 Nov 10;12:755708. doi: 10.3389/fpls.2021.755708

FIGURE 1.

FIGURE 1

Bacterial wilt evaluation in S. dulcamara and tomato cv. Marmande plants. Plants of the wild reservoir host S. dulcamara and tomato susceptible to bacterial wilt were root inoculated by soil soaking (A,C,E) or stem inoculated (B,D,F) with R. solanacearum UY031 carrying a luminescent reporter. (A,B) Wilting symptoms were recorded over time using a scale from 0 (no wilting) to 4 (completely wilted). n = 30–35 plants per plant species. (C,D) Bacterial concentrations in the stem at different time points from plants in panels (A,B), respectively. n = 4–8 plants per sampling day. (E,F) Bacterial content in relation to wilting symptoms for each plant individual analysed of the two species. n = 30 plant samples analysed for each species. Bacterial counts are expressed as log CFUs⋅g–1 tissue. ∗indicates statistical differences (p value < 0.05, T-student significant test). The experiments in panels (A,C) were repeated three times with similar results. The experiment in panels (B,D) was repeated twice with similar results.