FIGURE 2.

Bacterial colonisation and multiplication in stem-inoculated S. dulcamara and tomato cv. Marmande plants. (A) Non-destructive live luminescence imaging of four representatives S. dulcamara plants throughout a 30-day period after root inoculation with a luminescent R. solanacearum strain. Two symptomatic (A & F) and two asymptomatic (K & Q) plants are shown. Luminescent bacteria are detected in darker areas. Wilting symptoms (Disease index = 0–4) are indicated next to each plant inside the images. (B) R. solanacearum concentrations measured at the root, taproot, internode 1 (2–3 cm above the inoculation point) and 2 (6–9 cm above the inoculation point) in S. dulcamara plants uprooted 30 days post-inoculation (dpi) with the luminescent reporter strain. The results from asymptomatic (disease index 0) and symptomatic (disease index 1–4) plants are shown separately. Bacterial counts were calculated from tissue luminescence and are expressed as log CFUs⋅g^–1 tissue. ^∗indicates statistical differences (p-value < 0.05, T-student significant test).