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. 2021 Nov 10;12:755708. doi: 10.3389/fpls.2021.755708

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Copyright © 2021 Sebastià, de Pedro-Jové, Daubech, Kashyap, Coll and Valls.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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R. solanacearum distribution in stems of inoculated S. dulcamara and tomato cv. Marmande plants. (A) Representative luminescence imaging photographs at different wilting stages (Disease index 0–4) of stem sections from S. dulcamara (top panel) and tomato (bottom panel) plants stem-inoculated with luminescent R. solanacearum. Luminescent bacteria are detected as dark areas in transversal and longitudinal sections of plant internodes 1–4 organised bottom to top. Inoculation points are indicated by an arrow. (B) Representative fluorescence microscopy images of stem sections from S. dulcamara (top panel) and tomato (bottom panel) plants stem-inoculated with an R. solanacearum strain constitutively expressing GFP. Inoculations were performed at the base of the first true leaf and transversal sections obtained in the first internode, 2 cm above the inoculation point. Scale bars indicate 0.5 mm.