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. 2021 Nov 10;12:738056. doi: 10.3389/fimmu.2021.738056

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Copyright © 2021 Wang, Wu, Hsu, Pan, Shu and Cheng

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of apoptosis percentages by CD4+TIM3-low (CD4+TIM3-L) or CD4+TIM3-high (CD4+TIM3-H) subgroups, defined by average TIM3+ level. The figure shows (A) case demonstrations for apoptosis staining (Annexin V and Sytox Orange) and (B) scatter plots comparing apoptosis percentage on CD4+ or CD8+ T cells between CD4+TIM3-H or TIM3-L subgroups among healthy controls or patients with Mycobacterium avium complex-lung disease (MAC-LD). Apoptosis % was the sum of early apoptosis [Anexin V+/Sytox Orange–] and late apoptosis [Annexin V+/Sytox Orange+]. The cross bars in the scatter plots are median values, and the significance was analyzed by the Mann–Whitney U test. One star indicates p < 0.05.