Figure 4.

The percentages of cytokine production on CD4+ and CD8+ T cells from NTM-LD patients (N = 46) under stimulation with phorbol 12-myristate-13-acetate (PMA) plus ionomycin, plotted according to low or high levels of (A) CD4+ TIM3 and (B) CD8+TIM3, defined by the median levels. The cytokines, including IL-2, TNF-α, and IFN-γ, were measured by flow cytometry. Polyfunctional T cells were defined as positive for triple cytokines. The scatter plots are shown with cross bars of median levels. We analyzed the data with the unpaired t test. One star: p < 0.05; two stars: p < 0.01; and three stars: p < 0.001.