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Incorporation of IPA during viral RNA synthesis. (a) Phosphorylation of IPA and remdesivir in HEK293 cells (n = 3, data are mean ± SD). (b) Template RNA (black) used to measure the incorporation of ATP (A), IPA 5′-triphosphate (I), or remdesivir 5′-triphosphate (R). (c) RdRp-mediated RNA synthesis in the presence or absence of different nucleoside triphosphates. dATP, 3′-deoxy-ATP.
