Figure 2.

Kinetics of the interaction between Sporothrix and human monocyte-derived macrophages (hMDMs). (A) Sporothrix schenckii or (B) Sporothrix brasiliensis yeasts were made to interact with hMDMs at 37°C in a CO₂ incubator for 1, 4, and 18 h in medium containing human serum [whole (wHS) or inactivated (iHS)]. Mean ± SEM of the yeasts internalized by hMDMs after indicated interaction period for which at least 10 optical microscopy fields were analyzed; a minimum of 50 hMDMs were counted for internalized yeasts; three biological replicates, in duplicate, were performed. Statistical analysis was performed by ANOVA with Tukey’s multiple comparison posttest (****p < 0.0001, ***p < 0.001, and *p < 0.01).