Figure 4.

Hypoxia-induced exosomal miR-1246 upregulation mediates the functional differentiation of MDSCs

(A) The differentiation of MDSCs was measured after U87MG and P3 N-GDE and H-GDE stimulation. Antagomir-1246 impaired GDE-induced MDSC differentiation. (B) GDE-induced MDSCs were co-cultured with human peripheral CD8⁺ T cells from healthy volunteers at a 1:2 ratio. Three days later, CD8⁺ T cell proliferation was evaluated via flow cytometry by monitoring CFSE dilution. One representative experiment is shown. (C) IL-10 and TGF-β production was measured in N-GDE-, H-GDE-, and H-GDE+antagomir-1246-treated MDSCs. The data are presented as the mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.