Figure 1.

miR-122-5p was significantly upregulated in a mouse model of RBI and in patients with NPC after radiotherapy

(A) Procedures used to establish and validate RBI-model mice. (B) The escape latency of mice from the control and radiation groups (1 week after radiation) in the MWM test. n = 5. (C) The trajectory in the water maze of mice from the control and radiation groups on the fifth day of the MWM test. (D) The distance traveled in the target quadrant (%), the time (s) spent in the target quadrant, and the times the platform was crossed by mice from the control and radiation groups. (E) Pathological damage assessed using H&E staining. The sum area and the number of nuclei in the hippocampus of mice from the control and radiation groups (1 week after radiation). n = 3. Scale bars represent 200 μm for original magnification 20× (left panel) and 60× (right panel). (F) Screening of miRNAs in the hippocampus from the control and RBI-model mice. Significantly upregulated (or downregulated, p < 0.05) miRNAs were highly homologous between humans and mice (6 weeks after radiation, top-10 miRNAs). (G) The relative expression of these miRNAs in the hippocampus from control and RBI-model mice was measured using qRT-PCR (6 weeks after radiation). Data were normalized to U6. (H) The relative expression of miR-122-5p in patients with different stages of NPC (the same patients, n = 28, were divided into different tumor stages). Data were normalized to U6. Data are presented as the means ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.