Figure 3.

Neuronal damage induced by RBI was alleviated by the antagomiR-122-5p intervention

(A) H&E staining of the hippocampus from the different groups. The sum area and the number of nuclei in the hippocampus of mice (6 weeks after radiation). Scale bars represent 50 μm for original magnifications of 20× (top panel) and 40× (bottom panel). (B) βIII-tubulin-positive cells in the hippocampus and cortex from different groups (an important neuronal marker that is widely used in neuroscience to identify neurons). Scale bars represent 20 μm for original magnifications of 60×. (C) BrdU and NeuN double staining in neurons in the DG area of the hippocampus in the four groups. Scale bars represent 200 μm for original magnifications of 20×. Data are presented as means ± SEM, ns, not significant, ∗p < 0.05, ∗∗p < 0.01.