Figure 4.

Glial activation and neuroinflammation caused by irradiation were alleviated by the antagomiR-122-5p intervention

(A) The area and branch length of microglia in the hippocampus of different groups. Scale bars represent 50 μm for original magnifications of 20× (top panels), and scale bars represent 20 μm for original magnifications of 60× (bottom panels). The area and branch length were measured using Image-Pro Plus software. (B) The IOD of GFAP and the area of astrocytes in the hippocampus of mice from different groups. Scale bars represent 50 μm for original magnifications of 20× (top panels), and scale bars represent 10 μm for original magnifications of 60× (bottom panels). The area and IOD of astrocytes were measured using Image-Pro Plus software. (C–F) The levels of the inflammatory factors TNF-α (C), IL-1β (D), IL-6 (E), and S100B (F) in the hippocampus of RBI model mice at 6 weeks after intranasal administration. n = 6. Data are presented as means ± SEM, ns, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.