Figure 5.

The radiation resistance of the cell model of radiation injury was induced by the inhibition of miR-122-5p

(A) The viability of BV2 cells at different time points (0 h, 8 h, and 24 h) in the control, Rad (irradiation with 10 Gy), and Rad + inhibitor groups (irradiation with 10 Gy + miR-122-5p inhibitor). (B and C) Immunofluorescence staining for Iba-1 in microglia, the different numbers of beads phagocytosed by microglia (BV2) (B) and the branch length of microglia in the three groups (C). Scale bars represent 20 μm for original magnifications of 60×. (D) The area of BV2 cells, IOD of Iba-1 staining, perimeter, and Feret diameter of microglia (BV2) in the different groups. The data were measured using Image-Pro Plus software. (E) The TNF-α, IL-1β, and IL-6 levels in the supernatant from microglia in different groups (BV2, 8 h and 24 h) are shown. (F and G) A diagram illustrating the coculture of neurons with the microglia supernatant (F). The percentages of early apoptotic, late apoptotic and necrotic neurons after coculture with different microglia (BV2) supernatants (G). Data are presented as means ± SEM, ns, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.