Figure 8.

TNS1 may be related to the regulation of microglial polarization by the miR-122-5p intervention

(A) Screen of the miR-122-5p target gene by transcriptome data, TargetScan database, and miRwalk database. (B) Construction of the TNS1 dual-luciferase reporter gene and its potential sequence to which miR-122-5p binds. The relative luciferase activity of the miR-122-5p inhibitor and SIH groups expressing TNS1-WT and TNS1-MUT. (C and D) The relative expression of miR-122-5p and TNS1 in HMC3 cells (qRT-PCR) transfected with the miR-122-5p mimics (or scrambled mimics [SMs]) and miR-122-5p inhibitor (or scrambled inhibitor [SIH]). (E) The relative level of the TNS1 protein in different groups. Data were normalized to α-tubulin. Data are presented as means ± SEM, ns, not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.