Skip to main content
. 2021 Dec 2;2(12):100476. doi: 10.1016/j.xcrm.2021.100476

Figure 1.

Figure 1

Expansion of intermediate monocytes and plasmablasts is associated with COVID-19

(A) Schema highlighting the three clinical groups along with biological specimens obtained and assays performed.

(B) Flow cytometric analysis of immune cell frequencies in peripheral blood of healthy donors (one time point), non-COVID ARDS-affected individuals (day 1 ICU), and COVID-19-affected individuals (day 1 ICU). Comparisions with p values <0.05 by Wilcoxon rank-sum test are shown.

(C) Principal-component analysis (PCA) of immune cell frequencies displayed in (B).

(D) Weightings of immune cell frequencies that contribute to the PC1 embeddings in (C) and distinguish critically ill individuals (non-COVID ARDS/COVID-19) from healthy donors.

(E) scRNA-seq analysis of 98,327 cells showing the canonical immune lineages from peripheral blood of healthy donors (14,271 cells), non-COVID ARDS-affected individuals (13,060 cells), and COVID-19-affected individuals (78,922 cells).

(F) PCA performed by using differentially expressed immune cell gene modules (delineated with Arboreto; see Methods details) as molecular features of study participants in the three clinical groups.

(G) Weightings of the immune cell gene modules that are dominant contributors to PC1 and PC2 embeddings in (F). Flow cytometry analysis, 36 individuals (20 COVID-19-affected individuals, 6 non-COVID ARDS-affected individuals, and 10 healthy donors); scRNA-seq analysis, 79 distinct samples.