Skip to main content
. 2021 Dec 1;11:23246. doi: 10.1038/s41598-021-02681-6

Figure 3.

Figure 3

MPA and NET inhibit wound closure by endometrial stromal fibroblasts. (A) Representative images of endometrial stromal fibroblasts grown in 96-well plates scratched using an IncuCyte WoundMaker in the absence (control) or presence of MPA (100 nM) at 0, 12, 24, and 48 h at  × 10 magnification using the IncuCyte Zoom. Time of scratch is 0 h. The solid white line represents the fibroblast wound front. (B) Relative wound density of endometrial stromal fibroblasts grown in 96-well plates 24 h post-scratch in the presence (100 nM) or absence of MPA. Data shown is from a representative patient. Each symbol represents the mean + /− SEM from a single timepoint. (C) Wound closure of endometrial stromal fibroblasts grown in 24-well plates at 24 h post-scratch following treatment with 100 nM MPA, NET, LNG, or Progesterone for 48 h prior to scratch. MPA, NET, LNG, and Progesterone were maintained in cell culture media at 100 nM following scratch. Each symbol represents a single individual (n = 5). (D) Relative wound density of endometrial stromal fibroblasts grown in 96-well plates 24 h post-scratch following treatment with 100 nM MPA, NET, LNG, or Progesterone. Each symbol represents a single individual (MPA n = 10; NET n = 7; LNG n = 7; Progesterone n = 7). *p < 0.05.