TABLE 2.

Primers used for PCR amplification or sequencing

Primera	Sequenceb	Gene	Nucleotide positionsc
EV2	TCCGGCCCCTGAATGCGGCTAATCC	5′ NTR	446–470
EV1	ACACGGACACCCAAAGTAGTCGGTCC	5′ NTR	559–533
006	GGCAACTTCCACCACCACC	VP2	1197–1179
011	GCICCIGAYTGITGICCRAA	2A	3408–3389
012	ATGTAYGTICCICCIGGIGG	VP1	2951–2970
040	ATGTAYRTICCIMCIGGIGC	VP1	2951–2970
187	ACIGCIGYIGARACIGGNCA	VP1	2612–2631
188	ACIGCIGTIGARACIGGNG	VP1	2612–2630
189	CARGCIGCIGARACIGGNGC	VP1	2612–2631
222	CICCIGGIGGIAYRWACAT	VP1	2969–2951

^aEV1 and EV2 are from reference 19, 006 is from reference 5, and 011, 012, and 040 are from reference 15. 

^bSequences are shown 5′ to 3′, using standard IUB nucleotide ambiguity codes. I, deoxyinosine. 

^cNucleotide sequence coordinates are given relative to the sequence of PV1-Mahoney (GenBank accession no. J02281).