Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 1;11:23196. doi: 10.1038/s41598-021-02636-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assay protocol of the HISCL SARS-CoV-2 Antigen assay kit. The biotinylated SARS-CoV-2 Ag antibody (R1) was allowed to react with the sample at 42 °C for 3 min. Then, streptavidin-bonded magnetic particles (R2) were added and allowed to react at 42 ℃ for 2 min. After protein separation and washing, ALP-bound SARS-CoV-2 Ag antibody (R3) was added and reacted at 42 °C for 3 min. After another magnetic separation and washing, buffer solution (R4) and the chemiluminescent substrate (R5) were allowed to react at 42 °C for 5.5 min, then the luminescence intensity was measured. SARS-CoV-2: severe acute respiratory syndrome coronavirus 2; ALP: alkaline phosphatase; Ag: antigen. The illustration was drawn by A.K. using Microsoft PowerPoint 17.0 (Microsoft Corporation, Redmond, Washington: https://www.microsoft.com/en-us/microsoft-365/powerpoint).