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. 2021 Dec 1;12(12):1123. doi: 10.1038/s41419-021-04403-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A C57 mice were treated with a mixture of siSYK or siCtrl and mannose-conjugated JetPEI-polyplus (mannose-conjugated, JetPEI-polyplus) 4 h before the injection of CCL4 through the tail vein to blockade the transcription of SYK in liver macrophages. The mRNA level of liver SYK in siSYK or siCtrl groups was detected by qPCR 24 h after the injection of CCL4 (***P < 0.001); B Depleted Kupffer cells with CL before CCL4 injection, then detected SYK mRNA levels 24 h later (****P < 0.0001); C The level of SYK mRNA of siCtrl, siSYK and CL + siSYK groups after CCL4 injection (***P < 0.001); D And, 8 weeks after injection of CCL4, detected the expression of SYK by qPCR in siCtrl and siSYK groups (**P < 0.01); E Fibrotic liver was detected by photos, HE, masson, Sirius red staining and immunohistochemistry of α-SMA; F Determined the expression of TGF-β protein in the liver by Western blot; G The expression of α-SMA and Col1A1 in the liver by qPCR; H Immunofluorescence of α-SMA to detect the expression in liver tissue (magnification 100x).