Fig. 5. Blocking MoMFs SYK reduced the recruitments of Ly6Chi macrophages and the production of CXCL1.
A 12 h, 24 h, 48 h after the subacute lethal dose of TAA in mice, F4/80 and Ly6C expression were used to define the phenotypic transition pattern of macrophages. B On the 3rd and 7th day after TAA injection, HE and Sirius Red were induced to detect liver fibrosis. C mRNA levels of Col1A1, αSMA, in the liver at 1, 3, and 7 days after TAA injection; D, E Mice were injected with siCtrl or siSYK via tail vein 4 h before TAA injection. Flow cytometry analysis of the expression of CD11b, Ly6C and F4/80 in liver nonparenchymal cells. F mRNA levels of CCL5, CCL2, and CXCL1 in the liver at 1 and 3 days after TAA injection; G 24 h after TAA injection, the protein level of αSMA in different treatment groups was detected.