Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 1;12(12):1120. doi: 10.1038/s41419-021-04409-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Western blot analysis of FAP, α-SMA, and PDGFR-β proteins in isolated fibroblasts (n = 3). B Immunofluorescence analysis detected the expression of α-SMA and FAP in isolated fibroblasts. Scale bar, 50 μm. C Immunohistochemical staining analysis of α-SMA and FAP expression in breast cancer tissues and adjacent normal tissues. Scale bar, 200 μm. D MDA-MB-231 breast cells were co‑cultured with NF-CM or CAF-CM. The protein of MDA-MB-231 breast cells was extracted and detected the expression of β‑catenin, N-cadherin, MMP‑9, and MMP‑3 by western blot. E The effect of CAFs and NFs on the migration of breast cancer cells was analyzed by scratch experiment. F Transwell invasion assays analyzed the effection of CAFs and NFs on the invasion ability of breast cancer cells (n = 3). Scale bar, 30 μm. * indicates p < 0.05. The measurement data were expressed using mean ± SD, and the experiment was repeated three times; one-way ANOVA was used for multiple groups of data analysis.