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. 2021 Dec 1;12(12):1120. doi: 10.1038/s41419-021-04409-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Targetscan predicted that miR-18b combined with TCEAL7. B, C The target relationship between miR-18b and TCEAL7 verified by luciferase reporter gene assays. D Immunoprecipitation experiment to verify the binding of TCEAL7 and miR-18b (n = 3). E, F qRT-PCR showed that the expression of TCEAL7 was downregulated after co-cultured with CAFs-derived exosomes and NFs-derived exosomes (n = 3). G Western blot verified the results of qRT-PCR (n = 3). H Western blot observed the expression of TCEAL7 after transfected with miR-18b mimics and inhibitor in cancer cells (n = 3). I MDA-MB-231 cells are transfected with NC mimics+NC, miR-18b mimics+NC, or miR-18b mimics+pcDNA-TCEAL7 to detect invasion and migratory cell number by transwell. J Immunohistochemistry showed TCEAL7 expression in breast cancer tissues with different pathological grades (n = 3). * indicates p < 0.05. The measurement data were expressed using mean ± SD, and the experiment was repeated three times. Comparisons between two groups are analyzed by nonpaired t-test; one-way ANOVA was used for multiple data analysis groups.