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. 2021 Dec 1;11:23256. doi: 10.1038/s41598-021-02420-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunodetection of CFTR. (a) Cartoon showing locations of epitopes for the seven anti-CFTR antibodies used in this study. (b) Immunoblots of lysates after SDS-PAGE probed for CFTR using each antibody. BHK-WT cells were stably transfected with CFTR. Well differentiated primary bronchial epithelial (HBE) cells expressed endogenous CFTR. Protein (BHK-10 µg, HBE-40 µg) was detected after short and long exposures (Exp.1 and 2, respectively). Blots were cropped. (c) Confocal images of HBEs at the apical membrane after transduction with adenoviral mCherry-WTCFTR. Cells were cultured at the ALI for 10–15 days and immunostained on intact membrane support.