Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 1;11:23256. doi: 10.1038/s41598-021-02420-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The bases of cilia are immunostained using CFTR antibodies 596, 528, 769 and 217, but not 450, 570 and MM13-4. (a–g) Confocal images of well differentiated HBEs co-immunostained with CFTR antibodies 596, 528, 769, 217, 450, 570 or MM13-4 and the ciliated cell marker centrin-2. Arrows indicate ciliated cells. (h) Confocal images of cells coimmunostained with 596 and antibodies against either tubulin (left, ciliated cell marker), cytokeratin 14 (middle, basal cell marker) or MUC5AC (right, goblet cell marker). (i) Representative spatial cross-correlation functions calculated via ICCS analysis between centrin-2 and CFTR immunofluorescence using 596 (left) or 450 (right) measured from image sub-regions of interest (64 × 64 pixels). (j) Average apical immunofluorescence signals with different CFTR antibodies. Intensities were normalized to the excitation power (mean ± SD, n = 12–57, ***p < 0.0001, one-way ANOVA). (k) Colocalized fraction f_ICCS extracted from ICCS analysis (mean ± SD, n = 2–28, 450 vs 596, ***p < 0.0001; 217 vs 596, **p = 0.005; 769 vs 596, **p = 0.0059; 528 vs 596, *p = 0.0313), which indicates the arithmetic mean of the fractions of CFTR and centrin-2 antibody signals that interact.