Fig. 2.

Specificity and binding affinity of LXW7 ligand to ZDF-EPCs and expression of αvβ3 integrin on ZDF-EPCs

A. On-bead cell binding assay for testing the binding affinity and specificity of ligands to ZDF-EPCs showing high binding of ZDF-EPCs to LXW7 coated beads (right panel) compared to THP-1 monocytes (left panel). B. Quantification of the number of THP-1 monocytes and ZDF-EPCs bound to the LXW7 coated beads. C. Expression of αvβ3 integrin on ZDF-EPCs. D. Flow cytometry analysis of the binding affinity of LXW7 showing the blocking of its binding by anti-αvβ3 antibody. Scale bar = 100 μm. Data are expressed as mean ± SD. ****p < 0.0001, n = 3.