Fig. 9.

Neovascularization and collagen deposition evaluation of wounds treated by different scaffolds under ischemic condition.

A. Blood vessels stained with α-SMA (green) and RECA (red) in wound bed at day 14 post surgery. B–C. Immunostaining of collagen I and collagen III expression in wound bed at day 14 post surgery. D-F. Quantification of the blood vessel number/field (D) and immunofluorescence intensity of collagen I (E) and collagen III (F). Data are expressed as mean ± SEM. ****p < 0.0001, ***p < 0.001, *p < 0.05, n = 3 per group. Scale bar = 100 μm.