TABLE 1.
Regioselective decanoylation of polydatin catalyzed by various lipases.
| Enzyme | Source | V0 (mM/h) | Time (h) | C a (%) | OH b -regioselectivity c (%) |
|---|---|---|---|---|---|
| TLL | Thermomyces lanuginosus | 3.7 ± 0.1 | 9.0 | 35.4 ± 0.3 | 100 |
| Fe3O4-PDA-TLL | Thermomyces lanuginosus | 12.6 ± 0.3 | 14.0 | 98.7 ± 0.4 | 100 |
| TLIM | Thermomyces lanuginosus | 9.7 ± 0.2 | 14.0 | 94.1 ± 0.3 | 100 |
| RML | Rhizomucor miehei | 2.1 ± 0.1 | 6.0 | 20.5 ± 0.4 | 100 |
| CALB | Candida antarctica B | 3.8 ± 0.2 | 14.0 | 31.6 ± 0.5 | 100 |
| PEL | Penicillium expansum | 2.1 ± 0.2 | 6.0 | 17.3 ± 0.3 | 100 |
| ANL | Aspergillus niger | 2.4 ± 0.1 | 6.0 | 21.8 ± 0.4 | 100 |
| MML | Mucor miehei | 0.4 ± 0.1 | 6.0 | 11.1 ± 0.5 | 100 |
| Control | ― | n.d d | 4.0 | ― | ― |
The reactions were carried out at 55°C and 200 rpm by adding 0.03 mmol polydatin, 0.33 mmol vinyl decanoate, 180 mg enzyme into 3.0 ml anhydrous MeTHF.
a
Maximum substrate conversion.
b
Primary hydroxyl.
c
Regioselectivity was defined as the ratio of the concentration of the indicated product to that of all the products formed.
d
Not detected.