. 2021 Nov 18;12:779460. doi: 10.3389/fmicb.2021.779460

Table 2.

Nonsynonymous mutations observed in HSIV-P3 infectious molecular clones (IMCs).

Gene	HSIV-P3-114	HSIV-P3-161	HSIV-P3-284
Gag	K15R, E344G^, and T469I^	K15R	K15R, R387G^*
Pol	F8L, T27A, G113R, C317S, and G705K	F8L,T27A, G113R, C317S, and G705K	F8L, T27A, G113R, C317S, G705K, and N937S^*
Vif	E20G^*, M181I^a^,^#	M181I^a^,^#	M181I^a^,^#
Vpu	I42T
Env	L22P^, H105Q, K117R, G145E^#, R146G^#, I148T^#, R166K^#, K229E^#, A279V^#, Q308H^#, K341R, T371M^#, E427K^#, I489L^#, C530A^#, T531A^#, D545G^#, A576T, E732G^#, D756N^#, V780G, N807S^, and V827I^#	H105Q, K117R, G145E^#, R146G^#, I148T^#, R166K^#, K229E^#, A279V^#, Q308H^#, K341R, T371M^#, E427K^#, I489L^#, C530A, T531A, D545G^#, A576T, E732G^#, D756N^#, V780G, N814Y^*, and V827I^#,	H105Q, K117R, G145E^#, R146G^#, M147T^, I148T^#, R166K^#, K229E^#, A279V^#, Q308H^#, K341R, K346E^, T371M^#, E427K^#, I489L^#, C530A, T531A, D545G^#, A576T, E732G^#, D756N^#, V780G, and V827I^#
Rev	G65E, C89R, and G96R	G65E, C89R, and G96R	G65E, C89R, and G96R
Nef	R105K, S163N^#, M173I^#, V180M^*, R184K^#, and E201D	A53T^*, R105K, S163N^#, M173I^#, R184K^#, and E201D	R105K, S163N^#, M173I^#, R184K^#, E201D, and F203S^*

unique mutations to each of the IMCs.

mutations were observed in HSIV-vif biological isolates recovered from M08009.

M181L mutation introduced in SIV Vif changed to isoleucine in the recovered IMCs.