Skip to main content
. 2021 Sep 15;10:443–459. doi: 10.1016/j.bioactmat.2021.09.011

Fig. 8.

Fig. 8

Intra-articular injection of anti-integrin β1 or SCH772984 pretreated-IPFP-MSCs demolishes its survival and remedial effect of TE-IPFP-MSC suspension in ACLT rat model. (A) Schematic showing the intra-articular injection protocol of OA rat with ACLT surgery. Black arrows indicate time points when intra-articular injection was performed; green or red arrows indicates in vivo fluorescence imaging or histological sampling time. (B) Representative fluorescent in vivo image of OA rat knee joints over 3 days after intra-articular injection of anti-integrin β1 or SCH772984 pretreated DiR-IPFP MSCs. Rainbow color scale (Min: 1e5; Max: 1e6). (C) Quantitative analysis of area based on fluorescence intensity profiles (fluorescent in vivo imaging). (n = 6, **P < 0.01). (D) HE, safranin O-Fast Green, and COL 2 immunohistochemistry staining of rat knee joints. Images of femur and tibia were from black boxed area. (E) OARSI score (HE) and relative staining intensity (safranin O-Fast Green and COL 2 immunohistochemistry staining). (n = 6, *P < 0.05, **P < 0.01).