Fig. 2.

In vitro blood clot-scaffold interactions after 1 h of in vitro culture at 37 °C. Rabbit blood clot/scaffold cryosections (a) unstained or (b) SafraninO/Fast green-stained, (c) clot cell density (n = 6) and (d) % clot retraction (n = 3). Epifluorescent images of clot samples showing RITC-chitosan (red) and Hoechst 33258-stained cell nuclei (grey scale), for (e) PCL/clot, (f) 99-PCL/clot and (g) 83-99-PCL/clot with chitosan microparticles (arrowheads, g1 inset), clot cells (arrows, open arrowheads), and cell clusters at the edge (g2, inset). Symbols: open triangles: clot cells inside the pores, white arrows: clot cells near the scaffold edge, white arrowheads: 83% DDA chitosan microparticles. Scale bars: (a,b) 1 mm, (c,d) 200 μm, (e,f,g) 100 μm.