Fig. 4.
Hematoma phagocytes swarmed to (a-c) 83-99-PCL and not (d-f) 99-PCL scaffolds after 1 day in vivo. The red signal shows (a) 83% DDA or (d) 99% DDA chitosan and (b, e) matching non-specific green epifluorescence of the hematoma of the same field. Panels (c & f) show Toluidine blue/von Kossa stained hematoma below the scaffolds (PCL polymer is indicated by (*) where hematoma cells (blue stain) were displaced towards (c) 83-99-PCL and not (f) 99-PCL. Symbols: (a,b,d,e) open arrows: chitosan coating; * = PCL polymer; open arrowheads: detached chitosan coating; thin white arrows: phagocytes with internalized RITC-chitosan; (c,f) black arrows: hematoma cells. Scale bars: 250 μm.
