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. 2021 Sep 23;10:222–235. doi: 10.1016/j.bioactmat.2021.09.003

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Optical microscope images of TRAP stained osteoclasts on polished β-TCP cylinders with a Ca/P molar ratio lower than 1.500 (a and c) and greater than 1.500 (b and d). Scale bars represent either 100 or 200 μm. (e) TRAP Activity of cells after 24 h of cell culture experiment on all polished β-TCP cylinders from all batches (CaP1: Ca/P = 1.496, CaP2: Ca/P = 1.497, CaP3: Ca/P = 1.497, CaP4: Ca/P = 1.498, CaP5: Ca/P = 1.500 and CaP6: Ca/P = 1.502) with cell culture medium with no cells (NC), macrophages (MA) and osteoclasts (OC). Data were normalized by the mean of each cell experiment. Error bars stand for standard deviations (n = 5). Relative comparisons with the Bonferroni correction are indicated by: ***(p < 0.001) and NS (non-significant).