Table 7.
Inhibitory activity of the cold-pressed oils of apricot, cherry, peach, plum, and black cherry, tested at 9 μg/ml and 18 μg/ml, on the inhibitory capacity on mature biofilm by five pathogenic bacteria.
| MTT | CV | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Conc | AB | EC | LM | PA | SA | AB | EC | LM | PA | SA | |
| Apricot oil | 9 μg/ml | 0(± 0) | 0 (± 0) |
0(± 0) | 0 (± 0) |
0(± 0) | 19.82d (± 4.66) |
24.85d(± 2.27) | 1.15 (± 0.55) |
48.73d(± 0.65) | 40.90d (± 2.09) |
| Apricot oil | 18 μg/ml | 0(± 0) | 30.59d (± 1.06) |
0(± 0) | 0 (± 0) |
40.82d(± 0.98) | 27.22d (± 2.99) |
27.44d(± 1.73) | 33.68d (± 4.51) |
52.38d(± 4.56) | 48.22d (± 3.31) |
| Cherry oil | 9 μg/ml | 0(± 0) | 2.84 (± 0.20 |
0(± 0) | 7.93d (± 1.56) |
0(± 0) | 6.26c (± 1.19) |
24.33d(± 2.07) | 37.32d (± 2.42) |
33.00d(± 1.19) | 12.55d (± 1.03) |
| Cherry oil | 18 μg/ml | 0.50(± 0.02) | 22.61d (± 0.55) |
30.33d(± 1.51) | 38.71d (± 1.15) |
32.46d(± 0.40) | 27.40d (± 1.90) |
25.71d(± 2.19) | 47.02d (± 2.62) |
55.33d(± 4.15) | 72.88d (± 3.62) |
| Peach oil | 9 μg/ml | 25.48d(± 0.51) | 31.29d (± 0.97) |
0(± 0) | 20.41d (± 1.09) |
8.23d(± 1.19) | 13.92d (± 6.13) |
17.95d(± 1.19) | 11.96d (± 1.28) |
0(± 0) | 50.10d (± 2.09) |
| Peach oil | 18 μg/ml | 26.26d(± 0.73) | 44.21d (± 0.92) |
0(± 0) | 36.80d (± 1.99) |
18.06d(± 0.59) | 29.52d (± 1.70) |
25.80d(± 1.64) | 44.11d (± 2.06) |
56.36d(± 1.69) | 66.11d (± 2.06) |
| Plum oil | 9 μg/ml | 0(± 0) | 0 (± 0) |
0.83(± 0.15) | 6.38c (± 0.93) |
0(± 0) | 14.63d (± 1.40) |
24.94d(± 1.92) | 15.02d (± 1.19) |
34.26d(± 1.56) | 49.58d (± 2.04) |
| Plum oil | 18 μg/ml | 12.60d(± 0.94) | 42.64d (± 3.08) |
25.06d(± 0.40) | 16.69d (± 1.59) |
6.83d(± 0.28) | 17.44d (± 1.41) |
29.04d(± 1.27) | 40.60 (± 2.31) |
49.97d(± 0.85) | 52.72d (± 2.67) |
| Black cherry oil | 9 μg/ml | 19.96d(± 1.16) | 0 (± 0) |
0(± 0) | 0 (± 0) |
0(± 0) | 0 (± 0) |
0(± 0) | 4.91b (± 0.70) |
32.09d(± 1.69) | 0 (± 0) |
| Black cherry oil | 18 μg/ml | 44.36d(± 0.88) | 15.11d (± 0.62) |
30.80d(± 2.60) | 0 (± 0) |
21.51d(± 1.23) | 61.54d (± 0.79) |
31.03d(± 2.76) | 20.44d (± 1.78) |
56.48d(± 2.44) | 10.89d (± 0.88) |
Results are expressed as percentages (average ±SD) and calculated assuming the control (untreated bacteria, for which an inhibitory value= zero was assumed).
a: p < 0.1;
: p < 0.01;
: p < 0.001;
: p < 0.0001 compared with the control (ANOVA followed by Dunnett's multiple comparison test). AB, A. baumannii; EC, E. coli; LM, L. monocytogenes; PA, P. aeruginosa; SA, S. aureus; MTT, data of the inhibitory action exhibited by the oils on bacterial metabolism, evaluated through the MTT test; CV, data of the inhibitory action exhibited by the oils on the biofilm, evaluated through the Cristal violet assay.