Complete periodontal tissue regeneration in vivo. a) Micro-CT, HE and Masson's trichrome staining of periodontal tissue regeneration areas. White line displays defect boundary. D: dentin, PDL: periodontal ligament, AB: alveolar bone, S: scaffold, V: vessel, pointer: cementum. b) Semi-quantitative analysis of newly formed alveolar bone, cementum and periodontal ligament percentage in micro-CT and HE images. *: P < 0.05 versus Blank, #: P < 0.05 versus CGF, &: P < 0.05 versus CGF–DBBM, △: P < 0.05 versus Natural periodontium.